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. 2013 Nov 22;7:210. doi: 10.3389/fncel.2013.00210

FIGURE 4.

FIGURE 4

Anatomical evidence of gap junctions between MFs and pyramidal cell dendrites and thin-section TEM evidence for MF – pyramidal cell mixed synapses. (A,B) High-resolution 3D reconstructions (in two opposite z directions) of two dendrites of a lucifer yellow-injected pyramidal cell, dye-coupled to MFs (arrows). Double arrows mark a MF bouton. The arrowhead in (C) marks two dendrites with spines. (C) Image showing part of a dendrite (Dend) with dendritic spines (astrisks) and adjacent MFA . (D) Higher magnification of boxed area in (C). A typical large-diameter MF bouton containing synaptic vesicles surrounds a dendritic spine at a synaptic contact containing both close membrane appositions characteristic of gap junctions (white arrows) and wider membrane appositions with asymmetric dense cytoplasmic material (arrowheads) characteristic of postsynaptic densities. (E) Higher magnification of boxed area in (D) showing the gap junction contact (white arrow) between the MF bouton and the dendritic spine. A synaptic vesicle is marked with a black arrowhead (from Hamzei-Sichani et al., 2012).