Figure 3. Stage II Analysis of Autoantibody Reactivity and Corresponding Protein Levels.
The statistical methods (a) included analysis of variance (ANOVA) to take into account variation from multiple effects for each protein, filtering of interaction effects and post hoc Bonferroni correction to arrive at a set of three proteins, AKT3, FCGR3A and ARL8B. The box plots (b) illustrate the differences between the MDS patients and the healthy cohort for autoantibody reactivity to each protein of interest, with corresponding protein levels assessed by ELISA assays shown in (c). [The thickness of each box plot reflects the corresponding subject numbers (112 Healthy, 119 s-MDS, 42 t-MDS, 43 L) – see also Table 1b].
