(A) Transcriptional regulatory circuit within nine factors. Five factors (Nanog, Oct4, Sox2, Dax1, and Klf4) show auto-regulatory mechanism.
(B) Expanded transcriptional regulatory network showing target hubs of multiple factors within the previously identified protein interaction network in ES cells. Yellow circles represent nine factors we examined. The size of each circle reflects the degree of factor co-occupancy. Arrowhead indicates the direction of transcriptional regulation (A–B). Sox2, Klf4 and Myc were not in the original protein interaction network (Wang et al., 2006).
(C). Transcriptional regulatory circuit within 4 somatic cell reprogramming factors and Nanog