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. Author manuscript; available in PMC: 2013 Nov 22.
Published in final edited form as: Am J Reprod Immunol. 2013 Feb 8;69(5):10.1111/aji.12078. doi: 10.1111/aji.12078

Fig. 6.

Fig. 6

Absence of anti-HIV activity in vaginal epithelial secretions. Conditioned media were recovered after 48 hrs of culture and analyzed for inhibitory activity against BaL (a, c) and IIIB (b, d), a CCR5 and CXCR4 viral strain, respectively. Conditioned media were diluted 1:8 in complete media and incubated with virus at an multiplicity of infection of 1, for 1 hr at 37°C prior to incubation with TZM-bl cells. Vaginal epithelial cells were treated for 48 hrs with 5 × 10−8 M estradiol (E2) (a, b), and a panel of selective estrogen response modulators including Y134, PHTTP, ICI and tamoxifen (Tx) at 1 × 10−7 M (c, d). Each symbol on the graph represents a single patient sample. *P < 0.05 with respect to virus alone (BaL or IIIB).