Figure 6.

Effects of the BTA-EG₄ inhibitor of catalase-amyloid interactions on amyloid peptide toxicity in catalase overexpressing SH-SY5Y neuronal cells. (A) Human SH-SY5Y neuronal cells stably expressing the catalase gene vector (PCat) were pretreated with 0–20 μM BTA-EG₄ prior to exposure to medium alone (closed blue circles) or 50 μM Aβ (closed red circles) and cell viability determined by MTT reduction. Results are mean ± SEM; *P < 0.05 vs Aβ alone; one-way ANOVA. (B) Human SH-SY5Y neuronal cells stably expressing the catalase gene vector (PCat) were pretreated with 20 μM BTA-EG₄ (hatched columns) or medium alone (closed columns) and exposed to 25 μM Aβ 25–35 (clear columns), IAPP 20–29 (red columns), PrP 106–126 (blue columns), ABri 1–34 (green columns), or ADan 1–34 (yellow columns) and cell viability determined by MTT reduction. Results are mean ± SEM; *P < 0.05 vs amyloid peptide alone; one-way ANOVA.