Figure 4.

Currents activated by partial agonists are significantly enhanced by DTT. (a) Exemplary responses of R271C/Q226C α1 GlyR-expressing oocytes to 100 mM β-alanine alone (white bars) and 2 mM DTT (gray bars) or 500 μM propofol (hashed bars). Propofol was used only to confirm robust activation by β-alanine.¹¹ (b) Currents in response to 100 mM β-alanine (n = 4) and 100 mM taurine (n = 5) alone (white columns) and in the presence of DTT (gray columns) were normalized to the response to partial agonists alone (I/I_control); error bars indicate the SEM. Compared to the control response with a paired t test, *P < 0.05 and **P < 0.01.