. 2013 Aug 30;4(11):1469–1478. doi: 10.1021/cn400134p

Table 1. Nonlinear Regression Parameters for Glycine Activation of α1 GlyRs^a and Double-Mutant Cycle Analysis Results^b.

α1 GlyR	EC₅₀ (mM)	n_H	I_max (μA)	Ω^b
WT	0.04 ± 0.01	2.0 ± 0.1	6.5 ± 0.7
R271C	6.8 ± 0.47^c	1.5 ± 0.1	1.8 ± 0.3^c
Q226C	4.7 ± 0.59^c	1.6 ± 0.5	1.6 ± 0.7^c
R271C/Q226C	3.4 ± 0.67^c	1.3 ± 0.1	2.2 ± 0.4^c	4.3 × 10^–3

All values are means ± SEM from three to seven experiments.

The coupling coefficient, Ω, was calculated with the equation Ω = (EC_50,WT × EC_{50,R271C/Q226C})/(EC_50,R271C × EC_50,Q226C).

Significantly different from the WT value for that parameter (P < 0.001, ANOVA with Tukey post hoc analysis).

Table 1. Nonlinear Regression Parameters for Glycine Activation of α1 GlyRsa and Double-Mutant Cycle Analysis Resultsb.