FIGURE 4.

The Gal11p/MED15 KIX domain is required for Oaf1p- and ligand-dependent transactivation of peroxisomal genes and growth on oleic acid. A, the KIX domain of Gal11p/MED15 is necessary for the growth of yeast on the medium with oleic acid as a sole carbon source. The gal11/med15-deleted yeast strain was transformed with plasmids harboring either full-length Gal11p/MED15 or Gal11p/MED15 lacking the KIX domain (amino acids 1–100) or vector alone. Wild-type (WT) yeast was used as a positive control. The right panel shows yeast cells grown on normal YPD medium. B, the Gal11p/MED15 KIX domain is required for OA-induced transcription of the Oaf1p target genes FOX2 and FOX3. The yeast strains mentioned in A were used for this experiment. Quantitative real-time RT-PCR was performed in triplicates, and the mean values are shown. Error bars represent standard deviation.