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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Acta Trop. 2013 Aug 26;128(3):10.1016/j.actatropica.2013.08.012. doi: 10.1016/j.actatropica.2013.08.012

Figure 2. ELISA of mAbs against different F. hepatica antigens.

Figure 2

mAbs were tested against recombinant F. hepatica saposin like-protein 2 (FhSAP2), whole body extract (WE) from F. hepatica (FhWE), Schistosoma mansoni (SmWE), S. japonicum (SjWE) and Clonorchis sinensis (CsWE) using indirect ELISA. All mAbs were highly reactive with FhSAP2 (black bar) and FhWE (gray bar) with OD readings > 0.5. However, mAbs 1B2, 2C2 and 2H3 cross-reacted with extracts from S. mansoni, S. japonicum or C. sinensis. Dashed line represents the cut-off point (=0.2), which was calculated as the mean OD + 3SD of the normal mouse serum (NMS) or myeloma culture fluid (MCF).