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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Acta Trop. 2013 Aug 26;128(3):10.1016/j.actatropica.2013.08.012. doi: 10.1016/j.actatropica.2013.08.012

Figure 4. Immunolocalization of native FhSAP2 in F. hepatica adult worm.

Figure 4

(A) Cross-section of the middle part of the body of an adult fluke (×20) stained with supernatant of 2F5 mAb showing a thin line of fluorescence at the outermost part of the tegument (glycocalyx) (T) and intense staining toward the gut (G). Phalloidin-TRITC was used to counter-stain muscle (Mu). (B) A ×100 view of a tegument section showing strong fluorescence staining toward the tegumental cells (TC) underneath the tegument (T). (C) No staining was observed when mAb 2F5 was substituted by myeloma culture fluid.