FIGURE 5.

Kinetic analysis of E. coli adenylate kinase forward reaction at varying Mg²⁺ concentrations. In each panel, data points for fixed substrate concentrations of 1, 0.15, and 0.05 mm are plotted in open triangles, circles, and squares, respectively. Dotted lines show the fit of data to our newly proposed, magnesium activated random bi-bi model with competitive AMP and free ATP inhibition. With the implementation of our second order correction, we obtain an improved fitting, as demonstrated by the bold, dark lines.