TABLE 1.
Parameters generated from a global fitting of the magnesium activation model (Equation 9) to the AK forward assay data presented in Fig. 5.
| Parameter | Mg2+ activation model | Model with second order correction |
|---|---|---|
| kcat (s−1) | 605 ± 22 | 319 ± 28 |
| KAMP (mm) | 0.070 ± 0.005 | 0.207 ± 0.017 |
| KATP (mm) | 0.088 ± 0.006 | 0.049 ± 0.003 |
| KiT (mm) | 0.60 ± 0.12 | 0.28 ± 0.04 |
| koff/kon (mm) | 4.0 ± 1.5 (fixed) | 4.0 ± 1.5 (fixed) |
| κ ≡ ks/kon (mm) | NAa | 1.9 ± 0.3 |
| α | 8.6 ± 1.4 | 1.34 ± 0.13 |
| β | 52.6 ± 6.4 | 23.2 ± 3.0 |
| χ̃2 | 1.36 | 0.71 |
a
NA, not applicable.