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. Author manuscript; available in PMC: 2013 Nov 22.
Published in final edited form as: Cancer Res. 2012 Aug 8;72(19):10.1158/0008-5472.CAN-12-1248. doi: 10.1158/0008-5472.CAN-12-1248

Figure 2.

Figure 2

Serial passage of tamoxifen-resistant xenografts in mice resulted in metastasis. A, growth curve of OHTR100 cells grafted in Balb/c nude mice (n = 5) at initial transplant (OHTR100), 2nd (OHTR2nd), and 3rd passage (OHTR3rd). B, H&E stain of lung and liver sections of mice-bearing OHTR3rd primary tumors. C, morphological changes acquired by OHTR100 cells upon serial passage in mice. D and E, Western analysis of E-cadherin and vimentin (D), Hh signaling and target proteins (E) in OHTR cells quantified in the bar diagram. F, immunocytochemical analysis of SMO, GLI, and BMI1, with insets at higher magnification.