TABLE 1.

Gel filtration distribution of ³⁵S-apoA-I label in media after a 3 h incubation with RAW macrophages (see Fig. 4)

Peak #	Identityb	Distribution of 35S-apoA-I Labela
		(−) cAMP	(+) cAMP
		%
1	Intact 35S-apoA-I	73 (164 ng)	24 (54 ng)
2	Degraded (TCA soluble) 35S label	15 (34 ng)	27 (61 ng)
3	Intact 35S-apoA-I in cholesterol-containing particles	8 (18 ng)	48 (108 ng)
Totalc		96	99

apoA-I, apolipoprotein A-I.

^aThe percent distribution of label from the column runs shown in Fig. 5 was determined by summing the total counts in each fraction. For each experiment, peak 1 was defined as fractions 19–24, peak 2 as fractions 30–36, and peak 3 as fractions 10–18. The percent distribution is shown, along with the approximate mass of apoA-I that would be associated with each peak, given that 2,250 ng of apoA-I was initially used in the incubation (see Discussion)

^bThe state of apoA-I (intact, degraded, lipidated, etc.) was determined by Western blot analysis, TCA precipitation, and cofractionation with cholesterol as described in Results.

^cThe total did not add up to 100%, because there were small amounts of counts present in fractions that were not included in the three major peaks.