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. Author manuscript; available in PMC: 2013 Nov 22.
Published in final edited form as: Endocr Relat Cancer. 2013 Mar 22;20(2):10.1530/ERC-12-0342. doi: 10.1530/ERC-12-0342

Figure 4. MEK inhibition leads to lysosomal–mediated NIS protein degradation in MCF-7, SK-Br-3, and T47D breast cancer cells expressing exogenous NIS.

Figure 4

Western blot analysis showed that leupeptin prevented the decrease of NIS protein levels in U0126 treated MCF-7 cells (A), SK-Br-3 (B) and T47D (C) human breast cancer cells infected with recombinant adenovirus carrying hNIS (rAdFLhNIS). Cells infected with rAdFLhNIS were treated with U0126 for 24 hours, along with or without leupeptin for 8 hours. β-actin served as a loading control. The results are representative of two independent experiments. (D) Bar graph indicates the densitometry values of NIS protein level normalized with β-actin of cells under various treatments (represented as percentage relative to the corresponding untreated rAdFLhNIS transduced cells).