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Morphological alterations observed on both epimastigotes and trypomastigotes of Trypanosoma cruzi after 72 h of treatment with 50 µM of pepstatin A. Comparing with untreated epimastigotes, pepstatin A induced the swollen in cell body and detachment of the flagellum. In trypomastigotes the most evident effect was partial and whole flagellum detachment (arrowheads). k, kinetoplast; n, nucleus; f, flagellum.
