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Growth and differentiation of ED 16 foetal liver cells (FLC) before and after cryopreservation in vitro. (A) Cell recovery (percentage initially attached cells) during culture period before and after cryopreservation showed little loss of growth potential of cryopreserved FLC. (B) CK-18 staining 10 × 10. CK-18 positive cells were found in cultures of FLC after cryopreservation at day 3 in culture.
