Liver-specific CK-18 expression in the explanted neo-tissue at day 14 after transplantation. (A) Immunohistochemistry for CK-18 showed specific expression of this hepatocytic marker in the transplanted foetal liver cell groups (FLC, magnification 10 × 20). (B) RT-PCR analysis of specific gene expression within the neo-tissues confirmed CK-18 mRNA expression within the AV-loop transplantation group 14 days after transplantation (A1, A2). Legend: NC: Negative control without template; LS: Adult hepatocytes; Group A (A1, A2): transplantation of FLC in AV-loop; Group C (C1, C2): FLC transplanted in subcutaneous chamber; Group D (D1, D2): Subcutaneous groups without FLC.