Fig 2.

Inhibition of cell adhesion of transfected CHO cells on immobilized fibrinogen. Cells were transfected with different CD7/β₃ constructs and treated with different concentrations of doxycycline to regulate the CD7/β₃ expression level, which was measured by flow cytometry using a fluorescein-conjugated anti-CD7 antibody. Cell adhesion was determined by quantitative flow cytometry of cells in the supernatant and after trypsin treatment. The fusion-construct CD7(Δ215)β₃ resulted in strong inhibition of cell adhesion at low levels of expression, consistent with a dominant negative inhibition. The other fusion constructs resulted in inhibition that is dependent on the level of expression, indicating competitive inhibition.