Fig 3.
Loss of cytoskeletal anchorage of αIIbβ3 upon CD7(Δ215)β3 transfection. (A) Cells were transfected with αIIbβ3 and αIIbβ3+ CD7(Δ215)β3. Staining was performed with anti-αIIbβ3-FITC, anti-phalloidin-TRITC and anti-CD7-FITC. αIIbβ3-expressing cells adhere and spread on fibrinogen and the interaction between integrin and cytoskeleton is manifested by the localization of αIIbβ3 in adhesion plaques and the organization of the actin cytoskeleton in stress fibres that span between adhesion plaques. After co-transfection with CD7(Δ215)β3αIIbβ3-expressing cells do not form adhesion plaques and do not form stress fibres. (B) Quantification of cell adhesion of transfected CHO cells on fibrinogen. Cells were transfected with αIIbβ3 and αIIbβ3+ CD7(Δ215)β3. Adhesion of αIIbβ3-transfected CHO cells were set to 100% adhesion. Binding was significantly reduced by co-transfection with CD7 (Δ215)β3. Mean ± S.D. for n= 6 are given (***P < 0.001). (C) Quantification of cell adhesion of αIIbβ3-expressing CHO cells on fibrinogen under the influence of ReoPro (abciximab, 10 μg/ml). Adhesion of αIIbβ3-transfected CHO cells was set to 100% adhesion. Binding was significantly reduced by ReoPro. Mean ± S.D. for n= 3 are given (***P < 0.001).