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. 2008 Jul 9;14(1-2):290–302. doi: 10.1111/j.1582-4934.2008.00409.x

Fig 4.

Fig 4

VCAM-1, ICAM-1 and E-selectin mediated monocyte adhesion after treatment with CD7/VCAM-1, cytochalasin D, anti-VCAM-1, anti-VLA-4 and anti-E-selectin. Cells were transfected with either VCAM-1, ICAM-I, E-selectin, CD7/VCAM-1 or co-transfected. Expression of CD7/VCAM-1 was down regulated by doxycycline treatment. CD7/VCAM-1 effectively inhibits VCAM-1 but not ICAM-1 or E-selectin mediated monocyte adhesion. Adhesion was reduced after cytochalasin D treatment (50 ng/ml for 4 hrs) (A), anti-VCAM-1 (B) as well as anti-VLA-4 incubation (both 10 μg/ml for 15 min.)(C). Adhesion was reduced in ICAM-I transfected cells after cytochalasin D treatment but not after CD7/VCAM-1 co-transfection (D) and after incubation with anti-ICAM-I antibodies (E). Adhesion was reduced in E-selectin transfected cells after incubation with anti-E-selectin antibodies (F), but not after CD7/VCAM-1 co-transfection or cytochalasin D treatment (G). Mean ± S.D. for n= 6 are given (***P < 0.001).