Fig 5.

VCAM-1 and ICAM-1 mediated monocyte migration after treatment with CD7/VCAM-1, cytochalasin D and anti-VCAM-1. Cells were transfected with either VCAM-1, ICAM-I, CD7/VCAM-1 or co-transfected. Expression of CD7/VCAM-1 was down regulated by doxycycline treatment. Monocyte migration through VCAM-1-expressing CHO cells was inhibited by CD7/VCAM-1 (A), anti-VCAM-1 (B) and after cytochalasin D treatment (C). Migration of monocytes through ICAM-1-expressing CHO cells was not inhibited by transfection with CD7/VCAM-1, but cytoskeletal anchorage was essential for ICAM-1-mediated migration (D). Mean ± S.D. for n= 6 are given (***P < 0.001).