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. 2008 Jul 9;14(1-2):290–302. doi: 10.1111/j.1582-4934.2008.00409.x

Fig 6.

Fig 6

Monocytes adhesion, migration and rolling are inhibited by CD7/VCAM-1. Cells were incubated with buffer (non-stimulated) or stimulatedwith IL-1β (IL-1β-stimulated) and transfected with the vector alone (pTR-UF5 HUVECs) or the CD7/VCAM-1 fusion protein (CD7/VCAM-1 HUVECs). After IL-1β stimulation, monocyte adhesion and migration increased. (A) Adhesion could be significantly reduced after CD7/VCAM-1 transfection innon-stimulated and stimulated HUVECs. (B) For cell migration, cell activation is a prerequisite. On stimulated HUVECs, monocyte migration couldbe inhibited by the CD7/VCAM-1 fusion protein. Mean ± S.D. for n= 6 are given (**P < 0.01). HMECs in a flow chamber assay were stimulatedwith buffer or IL-1β and transfected with CD7/VCAM-1 fusion protein and/or blocked with an anti-VCAM-1 antibody (10 μg/ml for 15 min.). Increased amounts of adherent (C) and rolling monocytes (D) are detected after IL-1β stimulation. Transfection with CD7/VCAM-1, incubation with anti-VCAM-1 antibodies, and the combination of both result in a reduction of monocyte adhesion and rolling. Mean ± S.D. for n= 6 are given (**P < 0.01;***P < 0.001).