Fig 3.

The effect of catalase, a radical scavenger, MAFP, a non-specific phospholipase A₂ inhibitor, and AACOCF₃, a cytosolic phospholipase A₂ inhibitor on the H₂O₂-induced action on pacemaker currents of ICC from the murine intestine. (A) The effect of 1 mM H₂O₂ on pacemaker currents after pretreating cells with catalase (3000 units/ml). (B) Pacemaker currents exposed to 1 mM H₂O₂ after pretreating cells with 10 μM MAFP at a holding potential of −70 mV. (C) The effect of 1 mM H₂O₂ on pacemaker currents after pretreating cells with 10 μM AACOCF₃. (D, E and F) The inhibitory response to catalase, MAFP or AACOCF₃ on the H₂O₂-induced effects on pacemaker currents. The bars represent mean ± S.E. values (n= 6). Asterisks indicate significantly different from H₂O₂ alone (P < 0.05).