Fig 3.

Co-culture shifts differentiation to higher numbers of cell division. CD34⁺ cells were stained with CFSE and simultaneously cultured either without (grey dots) or with MSC (black dots) for 7 days. Each CFSE-peak (representing 0 to 12 cell divisions) was individually gated and analysed. Co-culture with MSC enhanced the number of cell divisions (A). Decrease of CD34 expression of CD133 and of CD38 expression was delayed to a higher number of cell divisions by co-culture with MSC. On the other hand, up-regulation of differentiation associated markers CD45, CD13 and CD56 shifted to higher numbers of cell division (B). Data of three independent experiments were normalized to the corresponding auto-fluorescence (red lines) and mean ± S.D. are demonstrated.