Table 2. Survival times of transgenic human PrP mice after inoculation of ovine prions*.
| Source code | Prion agent | Transmission data |
||||
|---|---|---|---|---|---|---|
| 129MM Tg35c mice |
129VV Tg152c mice |
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| Attack rate† | Survival, d‡ | Attack rate† | Survival, d‡ | |||
| AHVLA/SE 1919/0077 | Classical scrapie | 0/20 | 551, 551, 583, 615–666 (17) | 0/16 | 301, 344, 344, 364, 386, 428, 475, 519, 540, 543, 600–601 (6) | |
| AHVLA/SE 1919/0080 | Classical scrapie | 0/19 | 580, 586, 586, 620–666 (16) | 0/14 | 211, 336, 364, 364, 379, 519, 601–602 (8) | |
| FLI 1/06 | Classical scrapie | 0/15 | 426, 475, 628–728 (13) | 0/17 | 364, 497, 498, 517, 547, 559, 571, 595, 603–673 (9) | |
| FLI 83/04 | Classical scrapie | 0/15 | 270, 307, 311, 335, 349, 353, 635–672 (9) | 0/16 | 227, 300, 335, 440, 479, 510, 600–650 (10) | |
| FLI 107/04 | Classical scrapie | 0/17 | 382, 382, 459, 573, 574, 578, 606–636 (11) | 0/13 | 227, 228, 476, 606–706 (10) | |
| AHVLA/SE 1850/0001 | Atypical scrapie | 0/18 | 213, 332, 437, 537, 537, 621–656 (13) | 0/18 | 255, 318, 385, 397, 402, 403, 452, 453, 493, 518, 528, 538, 543, 552, 633–647 (4) | |
| AHVLA/SE 1850/0009 | Atypical scrapie | 0/18 | 440, 606–635 (17) | 0/15 | 293, 334, 403, 404, 419, 420, 426, 444, 584, 637–651 (6) | |
| FLI S7/06 | Atypical scrapie | 0/16 | 498, 610–659 (15) | 0/14 | 539, 545, 630–673 (12) | |
| FLI 14/06 | Atypical scrapie | 0/18 | 538, 540, 545, 572, 601–728 (14) | 0/15 | 313, 363, 489, 510, 592, 602–673 (10) | |
| FLI 26/06 | Atypical scrapie | 0/14 | 547, 553, 643–659 (12) | 0/14 | 435, 446, 554, 571, 608–673 (10) | |
| AHVLA/SE 1929/0877 | Ovine BSE | 0/16 | 315, 316, 348, 459, 557, 581, 620–659 (10) | 0/18 | 358, 363, 369, 382, 385, 440, 468, 476, 532, 550, 574, 600–602 (7) | |
| AHVLA/SE 1945/0032 | 2nd Passage ovine BSE | 1/19 | 337, 337, 434, 472, 517, 524, 616–661 (13) | 0/17 | 331, 331, 381, 386, 388, 388, 525, 527, 542, 562, 603–608 (7) | |
*PrP, prion protein; AHVLA, Animal Health and Veterinary Laboratories Agency; FLI, Friedrich-Loeffler-Institut; BSE, bovine spongiform encephalopathy. †All mice were inoculated with 30 μL of 1% (w/v) brain homogenate. Attack rate is defined as the total number of clinically affected and subclinically infected mice as a proportion of the number of inoculated mice. Subclinical prion infection was assessed by immunohistochemical examination of brain for abnormal PrP deposition and for recipients of AHVLA inocula by sodium phosphotungstic acid precipitation of 250 μL 10% brain homogenate and analysis for PrPSc by proteinase K digestion and immunoblotting. ‡The interval between inoculation and culling because of intercurrent illness, senescence, or termination of the experiment in days. Death dates of individual mice are shown together with the range for mice surviving beyond 600 d with the number of mice in this range shown in parentheses. Mice culled with postinoculation periods of ≤200 d due to intercurrent illness (all confirmed negative for prion infection) were not included in calculating attack rates.