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. Author manuscript; available in PMC: 2013 Nov 22.
Published in final edited form as: Immunol Cell Biol. 2012 Sep 25;90(10):10.1038/icb.2012.44. doi: 10.1038/icb.2012.44

Figure 3. Molecular knock-down of human ADAM28 protein levels diminishes TNF-α shedding in HEK293 cells.

Figure 3

HEK293 cells were co-transfected with TNF-α and ADAM28 vectors in addition to either scrambled siRNA or three different human ADAM28 (A28) siRNA. After 48hrs, ADAM28 mRNA (A), ADAM28 protein (B, C and D), TNF-α shedding (E; top panel) and membrane bound pro-TNF-α protein levels (E; bottom panel) were determined. Data expressed as mean ± SEM (A, C, D and E). *p<0.05 compared to scrambled siRNA (scrambled); n=3–6 from at least 2 independent experiments.