Table 2.

Probes and corresponding taxonomic affiliations indicative of the active nest area and reference soil with a high probability (P ≤ 0.05), revealed by indicator species analysis^a

Sampling site, methanotroph type, and probe (taxonomic affiliation)	Indicator value
Active nest area, type I
P_JR3.505 (upland grassland soil cluster)	1.00
O_501.286 (Methylococcus-like)	0.97
P_JR3.593 (upland grassland soil cluster)	0.94
O_BB51.299 (Methylobacter)	0.91
P_ML_SL.3.300 (Methylobacter)	0.91
LF1a.456 (Methylobacter-like)	0.91
DS2.287 (deep sea cluster)	0.90
Ib453 (type Ib, general)	0.87
P_Mb_LW12.211 (Methylobacter)	0.85
LP21.436 (pmoA2)	0.85
Kuro18.205 (deep sea cluster)	0.73
P_LK580 (Lake Konstanz sediment cluster)	0.72
Reference soil
Type I
P_OSC220 (Finnish soil clones)	0.98
P_MmES543 (Methylomonas)	0.96
LW14.639 (Methylosarcina-like)	0.86
Alp7.441 (Methylomonas-like)	0.85
P_JRC3.535 (Japanese rice cluster)	0.83
JHTY1.267 (Methylogaea-like)	0.82
P_Mb_C11.403 (Methylobacter)	0.78
MsQ290 (Methylosarcina-like)	0.78
O_fw1.641 (Methylococcus-like and Methylocaldum-like)	0.56
Type II
O_II509 (type II, general)	0.87
P_McyM309 (Methylocystis)	0.74
P_Mcy270 (Methylocystis)	0.63

^aOnly data for probes targeting methanotrophs are shown.