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Primer combinations used for detection of methanotrophs
| Primer set | PCR or methodology | Target gene | Target microorganism | Reference(s) |
|---|---|---|---|---|
| mmoXLF/mmoXLR | Nested PCR | mmoX | Methylocella genus specific | 31 |
| A189_b/cmo682 | Nested PCR | pmoA | “Candidatus Methylomirabilis oxyfera” specific (phylum NC10) | 32, 54 |
| Cmo182/cmo568 | ||||
| V170f/V613b | Direct PCR | pmoA | Methylacidiphilum specific (phylum Verrucomicrobia) | 33 |
| A189f/T7_A682r | Diagnostic microarray analysis | pmoA | Aerobic methanotrophs (general probe) | 4, 27 |
