Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Ralstonia sp. | ||
| T6 | Wild type, TCP degrader, Gm− Tcr | Lab stock |
| T6-ΔtcpA | tcpA insertion mutant of T6 | This study |
| T6-ΔtcpA-com | Complementation for the tcpA insertion mutant of T6 | This study |
| Cupriavidus necator JMP134 | Wild type, 2,4,6-trichlorophenol degrader | 24 |
| E. coli | ||
| DH5α | Host strain for cloning vectors | Lab stock |
| DH10B | Host strain for cloning vectors | Lab stock |
| SM10λpir | Conjugation strain | Lab stock |
| BL21(DE3) | Host strain for expressing vectors | Lab stock |
| Plasmids | ||
| pMD19-T | T-A cloning vector, Ampr | TaKaRa |
| pMD19-RXA | Vector for tcpRXA heterologous expression | This study |
| pMD19-RXA-JMP134 | Vector for heterologous expression of tcpRXA from C. necator JMP134 | This study |
| pUC19 | Parent vector of pMD19-T digested with EcoRV | TaKaRa |
| pJQ200SK | Suicide vector, Gmr | 22 |
| pJQ-TY1 | Vector for the tcpA insertion mutant of T6 | This study |
| pRK2013 | Helper plasmid, mob+ tra+ Kmr | Lab stock |
| pSC123 | Vector for transposon-mediated mutagenesis of bacteria, Kmr | 25 |
| pBBR1MCS-2 | Broad-host-range cloning vector, Kmr | 23 |
| pBB-XA | pBBR1MCS-2 derivative carrying tcpX and tcpA genes | This study |
| pET-29a(+) | Expression vector, Kmr | Lab stock |
| pET-I | pET-29a(+) derivative carrying dhpI | This study |
| pET-J | pET-29a(+) derivative carrying dhpJ | This study |