Fig 3.
Zymogram analysis of the active chitinases in culture supernatants. Strain FPU-7 was grown at 30°C for 2 days with shaking in polypeptone medium supplemented with Glc, GlcN, GlcNAc, or (GlcNAc)2 as described in Materials and Methods. Aliquots of culture supernatants were loaded onto an SDS-PAGE gel containing 0.1% (wt/vol) ethylene glycol chitin, and activity staining was performed. Medium alone served as a negative control; unsupplemented cultures (polypeptone medium alone) and cultures supplemented with Glc, GlcN, GlcNAc, and (GlcNAc)2 were tested.