Fig 1.

G. europaeus pyrE locus, aldC gene cluster, and putative acetoin pathway predicted from draft genome sequences of the type strain, LMG18890^T. (A) Gene arrangement of pyrE encoding orotate phosphoribosyltransferase on contig 26 (CADP01000026.1). The arrows above and below the scheme indicate primers used for the construction of a pyrE disruption vector and a pyrE marker cassette, respectively (Fig. 3). cs, citrate synthase; gr, glutamate racemase. (B) The aldC gene cluster located on contig 6 (CADP01000006.1). The arrows above and below the scheme indicate primers used for the construction of als and aldC disruption vectors, respectively (Fig. 3). aldC, α-acetolactate decarboxylase; als, α-acetolactate synthase; h, hypothetical protein; ldh, lactate dehydrogenase; pdc, pyruvate decarboxylase. (C) Putative acetoin pathway in G. europaeus. AHAS, acetohydroxyacid synthase; AldC, α-acetolactate decarboxylase; Als, α-acetolactate synthase; DR, diacetyl reductase; Ldh, lactate dehydrogenase; Pdc, pyruvate decarboxylase.