Table 2.
Primers used in this study
| Primer | Sequence (5′–3′)a | Use |
|---|---|---|
| EU-F | GGAATTCGATCGCCATCCACGACGAAT | pyrE disruption |
| ED-R | CGGGATCCAGCCCGGAAAACATTCAGCA | pyrE disruption |
| E5′-R | GGAGCCTGTTGAAAGTCCAG | pyrE disruption |
| E3′-F | GAAGAAGCACTGGCGCTGAA | pyrE disruption |
| EP-F2 | CTGCCATATCCCGTGTTCGT | pyrE marker cassette |
| E-R4 | TCGCCATAGGGAAAGACTGC | pyrE marker cassette, genotyping |
| EP-F3 | ATCCCCACCAGCATGTTCAC | Genotyping |
| aldC-F | AGAATTCGATCACGCTCGAAACCCTGT | aldC disruption |
| aldC-R | AAATCGATCGATATCCCCCACCAGTTCA | aldC disruption |
| aldC-i1 | ATTCACGAAGCCATTCGCGTGGCTG | aldC disruption |
| aldC-i2 | GATTGCCCGAGAATGGTGAAGCAGG | aldC disruption |
| aldC-F2 | CCTGAACCTTCATTTCAATGGTGCG | Genotyping |
| aldC-R2 | GTCCATGCTCTGGTGCGTAAGCTTC | Genotyping |
| als-F2 | AAATCGATAGGAGACTCAGGTCTTGAAGCTGAA | als disruption, genotyping |
| als-R2 | AAGGATCCGTTCCAGTTGATGCTGGATTCG | als disruption |
| als-i1 | AATCACCTGCTGATGAAGCCGCTG | als disruption |
| als-i2 | GTCAAACAGGCGGTCCACCTTC | als disruption |
| ldh-i2 | AGTACACGCCACTGTTCCACCAG | Genotyping |
| pyrE-probe-F1 | CCTTCATCTATCCGCGCAAC | Probe for Southern blot analysis |
| pyrE-probe-R1 | ACAGGTCGCCTGCATGATCT | Probe for Southern blot analysis |
| aldC-probe-F1 | AATGTGCCACAGGACGAGGT | Probe for Southern blot analysis |
| aldC-probe-R1 | TGAAGGTTCAGGACGACACG | Probe for Southern blot analysis |
a
Restriction enzyme sites are underlined.