Fig 4.

Killing kinetics of L. monocytogenes persisters treated with gentamicin and carbohydrates or by nitrofurantoin. (A) Gentamicin killing of persisters with different fermentable carbohydrates. The results obtained for gentamicin in IMM (■, full line), gentamicin plus 10 mM glucose in IMM (◆, full line), gentamicin plus 10 mM fructose in IMM (Δ, full line), gentamicin plus 10 mM mannitol in IMM (□, broken line), gentamicin plus 10 mM glycerol in IMM (●, full line), and the growth control of 10 mM glucose in IMM (◆, broken line) are shown. The experiment was performed with two biological replicates, and error bars indicate the standard deviations. (B) Time-dependent killing of L. monocytogenes EGDe (▲) with 400 μg of nitrofurantoin ml⁻¹ in either BHI (full line) or peptone saline (broken line). The experiment was performed with three biological replicates, and error bars indicate the standard deviations. At 48- and 72-h time points, only two biological replicates were performed.