Fig 2.

Biotransformation of 4CA and the formation of a transformation product in PAO1. (A) Biotransformation of 4CA (■; initial concentration, 2 mM) by PAO1 using a resting-cell technique and the formation of 4-chloroacetanilide (4CD; ○) as a transformation product. (B) LC-MS spectra of the transformation product obtained from the PAO1 culture supernatant of medium containing 4CA after 15 h of incubation. The m/z value was 168.02, which indicates that 4CD (C₈H₇ONCl) is the transformation product. (C) Biotransformation of 4CD (○; initial concentration, 2 mM) by PAO1 using a resting-cell technique.