Table 2.
Chemotaxis and growth of PAO1 and the ΔCTPL on chloroanilines and aromatic analogues
| Compounda | Chemotactic responseb |
Cell growthc | |
|---|---|---|---|
| PAO1 | ΔCTPL | ||
| Aniline | − | − | − |
| 2-Chloroaniline (2CA) | − | − | − |
| 3-Chloroaniline (3CA) | + | − | − |
| 4-Chloroaniline (4CA) | + | − | − |
| 2,4-Dichloroaniline (24DCA) | − | ND | − |
| 3,4-Dichloroaniline (34DCA) | + | − | − |
| 4-Chloroacetanilide | − | ND | − |
| Catechol | ++ | − | + |
| 4-Chlorocatechol | + | − | ND |
| Sodium salicylate | − | ND | ND |
| Anthranilic acid | + | − | + |
| 4-Aminobenzoic acid | + | − | − |
| Sodium benzoate | + | − | + |
| 4-Hydroxybenzoic acid | − | ND | + |
| Nitrobenzene | − | ND | ND |
| 4-Chloronitrobenzene | + | − | ND |
| Benzene | − | ND | ND |
| Toluene | − | ND | ND |
| Casamino Acids (positive control) | +++ | ++ | + |
| HEPES (10 mM, negative control) | − | − | − |
| H2O (negative control) | − | − | − |
The concentration of the test compound was 1 mM for the chemotaxis test and 10 mM for growth determination.
The chemotactic response was measured by a computer-assisted capillary assay as described in Materials and Methods. Cell numbers (N) in the analysis frame area were captured at a starting time (t0) and a 60-s chemical exposure time (t60). The strength of chemotactic attraction is shown by the increase in cell number in the analysis frame area at t60 after normalization with that at t0 (Nt60/Nt0). The value of the normalized cell number is represented by the symbols, as follows: 4 ≥ +++ > 3; 3 ≥ ++ > 2; 2 ≥ + > 1.2; 1.2 ≥ − > 0; and ND, not determined.
Cell growth in MSB medium was determined as the optical density of the cells at 600 nm (OD600) after 24 h of incubation. The cell optical density value is represented by the symbols, as follows: ++, >0.6; 0.6 ≥ + > 0.1; 0.1 ≥ − > 0 (no growth); and ND, not determined.