Fig 3.

Alleviation of CVB3-induced myocarditis by pHMGB1 coimmunization. (A) One representative heart section is shown for 5 mice per group (magnification, ×100). (B) Myocarditis was assessed as the percentage of the heart section with inflammation, compared with the overall size of the heart section, with the aid of a microscope eyepiece grid. Each group contained 5 mice. ∗, P < 0.05. (C) Survival rates of mice were observed until day 28 following challenge with a lethal dose of CVB3 (5 times the LD₅₀). Each group contained 10 mice. Experiments were repeated three times with similar results. All of the experimental data were pooled and are presented together. ∗, P < 0.05; ∗∗∗, P < 0.001, versus the mock-immunized group.