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Tryptophan fluorescence and thermal denaturation of rPA bound to Alhydrogel. (A) rPA adsorbed to nonmodified and 50% and 100% phosphate-saturated Alhydrogel. (B) rPA on Alhydrogel with 1 to 10 mM free phosphate. The thermal transition of nonadjuvanted rPA (solid line) occurred at 48.5°C. All curves were normalized to values of 1 at 25°C and 0 at 65°C.
