Phylogenetic tree depicting the relationships of the rickettsiae in Ixodes angustus with other species of Rickettsia based on neighbor-joining analyses of concatenated sequence data of five genes (17-kDa gene, ompA, gltA, 16S rRNA gene, and sca1). SFG, TG, and TRG refer to the spotted fever group, typhus group, and transitional group of Rickettsia, respectively. We have not included R. helvetica within the SFG based on the findings of a recent study (11) that considered the position of this species within the genus Rickettsia as incertae sedis. The scale bar represents the inferred substitutions per nucleotide site. The relative support for clades in the tree produced from the NJ and MP analyses are indicated above and below branches, respectively.