Functional activity of heterologous CB1190 THF MO expression clones. (A) THF removal by RHA1 containing plasmid pTip-CB1190-thfmo (■), the empty vector pTip-QC2 (control) (□), and abiotic samples (○). (B) Removal of dioxane and accumulation of HEAA by plasmid pTip-CB1190-thfmo clones, (■, dioxane; ▲, HEAA), empty vector pTip-QC2 clones (□, dioxane; △, HEAA), and abiotic samples (○, dioxane; ×, HEAA). Error bars indicate standard deviations. All conditions were run in triplicate.