Table 3.
Phenotypes and cpa rtPCR profiles of putative C. perfringens isolates found on mCP agar from river water samplesa
| Sample | Reference strain | Phenotype on mCP agar | C. perfringens cpa rtPCR result | 16S rRNA gene-based identification |
|---|---|---|---|---|
| 5 | CCRI-20251 | Pink | − | C. perfringens |
| CCRI-20253 | Pink | + | NA | |
| CCRI-20304 | Pink | + | NA | |
| CCRI-20305 | Pink | − | C. sardiniense | |
| CCRI-20306 | Pink | + | NA | |
| CCRI-20307 | Colorless | − | NA | |
| CCRI-20308 | Colorless | + | C. perfringens | |
| CCRI-20309 | Colorless | − | NA | |
| CCRI-20310 | Colorless | + | C. perfringens | |
| CCRI-20328 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20313 | Pink | + | NA | |
| CCRI-20314 | Pink | + | NA | |
| CCRI-20315 | Pink | + | NA | |
| CCRI-20316 | Pink | + | NA | |
| CCRI-20317 | Pink | + | NA | |
| CCRI-20318 | Pink | + | NA | |
| DNG | Pink | ND | NA | |
| DNG | Pink | ND | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20339 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20324 | Colorless | − | NA | |
| CCRI-20325 | Colorless | − | NA | |
| CCRI-20326 | Colorless | − | NA | |
| CCRI-20327 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20329 | Colorless | − | NA | |
| CCRI-20330 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20335 | Colorless | − | NA | |
| CCRI-20336 | Colorless | − | NA | |
| CCRI-20337 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20329 | Colorless | − | NA | |
| CCRI-20342 | Colorless | − | NA | |
| CCRI-20343 | Colorless | − | NA | |
| CCRI-20344 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20349 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20353 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20356 | Colorless | − | NA | |
| DNG | Colorless | ND | NA | |
| CCRI-20358 | Pink | + | NA | |
| CCRI-20359 | Pink | + | NA | |
| CCRI-20360 | Colorless | − | NA | |
| CCRI-20361 | Colorless | − | NA | |
| CCRI-20362 | Colorless | − | NA | |
| CCRI-20363 | Colorless | − | NA | |
| CCRI-20364 | Colorless | − | NA | |
| CCRI-20365 | Colorless | − | NA | |
| CCRI-20366 | Colorless | − | NA | |
| CCRI-20367 | Colorless | − | NA | |
| CCRI-20368 | Colorless | − | NA | |
| CCRI-20369 | Colorless | − | NA | |
| CCRI-20370 | Colorless | − | NA | |
| CCRI-20371 | Colorless | − | NA | |
| CCRI-20372 | Colorless | − | NA | |
| CCRI-20373 | Colorless | − | NA | |
| CCRI-20374 | Colorless | − | NA | |
| CCRI-20375 | Colorless | − | NA | |
| CCRI-20376 | Colorless | − | NA | |
| CCRI-20377 | Colorless | − | NA | |
| CCRI-20378 | Colorless | − | NA | |
| CCRI-20379 | Colorless | − | NA | |
| CCRI-20380 | Colorless | − | NA |
a
The identification of discordant strains (mCP+/rtPCR− or mCP−/rtPCR+) was done by 16S rRNA gene sequence analysis. CCRI, culture collection of Centre de Recherche en Infectiologie de l'Université Laval; NA, not applicable; DNG, did not grow upon isolation attempt; ND, not done.