Fig 5.

Thermal stability of the MetE mutants. (a) The WE (filled circles), WE-214 (open circles), WE-CA (filled triangles), and WE-214A (open triangles) strains were grown in M9 glucose medium in an automatic growth-measuring incubator at 44°C. The averages of two independent experiments are presented. (b) Serial dilutions of cultures growing logarithmically at 37°C in M9 glucose medium (OD₆₀₀ of 0.5) were spotted onto M9 glucose or M9 glucose supplemented with l-methionine (50 μg ml⁻¹) agar plates. The cells were then incubated for 24 h at 44°C.