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Combined effects of mutated MetA and MetE proteins on E. coli growth in the presence of acetate or at elevated temperature. The WE (filled circles), Y229 (open circles), WE-214A (filled triangles), and Y229-214A (open triangles) strains were grown in M9 glucose medium (pH 6.0) supplemented with sodium acetate (20 mM) at 37°C (a) or in M9 glucose medium at 45°C (b) in an automatic growth-measuring incubator. The averages of two independent experiments are presented.
