Fig 5.

REGIII-γ protein expression in ileum and colon of GF and B. breve- and E. coli-monocolonized mice. B. breve-monocolonized mice showed higher REGIII-γ protein expression than E. coli-monocolonized mice. (A) Representative immunofluorescence staining (anti-REGIII-γ; red) in each group. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). (B) Quantification of REGIII-γ staining intensity was expressed as fold increase relative to the mean of GF mice, which is set to 1. Each data point represents an individual mouse. Data were pooled from two to three independent experiments. P values are indicated on the figure and were calculated using ANOVA, followed by a Bonferroni post hoc test.