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KsgA deficiency confers susceptibility to high osmolarity in S. Enteritidis. Growth of the S. Enteritidis ksgA::Tn5, ksgA::Tn5-pACYC184-ksgA, and WT parent strains was assessed in LB broth and 1.1 M NaCl with 30 μg/ml of nalidixic acid for up to 6 days at 42°C. Mean log10 CFU values ± standard deviation (SD) per ml were determined on XLD agar at different time points for two independent replicates.
