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KsgA deficiency confers resistance to kasugamycin (KSG) in S. Enteritidis. Growth was monitored under increasing concentrations of KSG (50 to 1,000 μg/ml) in LB broth by measuring OD600. The average MIC of KSG was 500 μg/ml, 150 μg/ml, and 100 μg/ml for the ksgA::Tn5, ksgA::Tn5-pACYC184-ksgA, and WT strains, respectively.
