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KsgA-deficient S. Enteritidis reduces the ability to survive within chicken macrophage HD11 cells. A gentamicin protection assay with an MOI of 20 was performed at 42°C. Mean log10 CFU values ± standard deviation (SD) per ml were determined at 30 min (invasion) and 8 h (survival) postinfection. The uptake of the ksgA::Tn5 mutant (a) was significantly higher than that of the wild-type parent strain (b) (P < 0.05). Three independent replicates were included in these experiments.
