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. 2013 Dec;57(12):6254–6264. doi: 10.1128/AAC.01703-13

Table 3.

Steady-state kinetic parameters for incorporation of the triphosphate forms of the 4′-substituted compounds by HIV-1 RT

dNTP Km (μM)a kcat (min−1)a kcat/Km (min−1 · μM−1) Fold change in incorporation efficiencyb
dATP 2.35 ± 0.06 0.66 ± 0.13 0.3 1
EFdA-TP 0.27 ± 0.01 3.47 ± 0.04 12.9 45.9
EdDAP-TP 0.15 ± 0.01 2.55 ± 0.10 17.0 60.7
MedA-TP 0.29 ± 0.01 1.24 ± 0.05 4.3 15.3
EtdA-TP 0.88 ± 0.19 3.41 ± 0.12 3.9 13.9
dTTP 0.60 ± 0.01 1.04 ± 0.12 1.7 1
MedT-TP 0.68 ± 0.22 1.58 ± 0.15 2.3 1.3
EtdT-TP 2.00 ± 0.22 1.44 ± 0.20 0.7 0.4
a

Values are means ± standard deviations of two independent experiments and were determined from the Michaelis-Menten equation using GraphPad Prism 4.

b

Fold change in incorporation efficiency is the ratio of the incorporation efficiency (kcat/Km) of NRTI-TP over that of dATP or dTTP [(kcat/Km)NRTI-TP/(kcat/Km)dATP or (kcat/Km)NRTI-TP/(kcat/Km)dTTP].