Fig 2.
PCR scanning analysis of pETB plasmids. The gene organization of the acquired region in the pETBTY825 plasmid was examined using PCR scanning analysis. Various combinations of the 14 primers that target the selected seven genes were used. A schematic view is shown in Fig. 1B. The results of the PCR analysis of regions 1 to 7 are shown. By comparing the length of each amplified fragment with that from pETB, the regional heterogeneity was determined. Results with pETB from the following strains are shown in the indicated lanes: 1, TY4; 2, TY825; 3, TY632; 4, TY1020; 5, TY1603; and 6, TF3056.