Table 1.

Oligonucleotides used for PCR amplification

Purpose and gene or region	Primer	Oligonucleotide sequence (5′-3′)	Product size (bp)	Primer design reference or source
PCR
etb	ET-3	ATACACACATTACGGATAAT	629	13
	ET-4	CAAAGTGTCTCCAAAAGT
aac(6′)/aph(2′)	aac/aph-F	TACAGAGCCTTGGGAAGATG	406	32
	aac/aph-R	CATTTGTGGCATTATCATCATATC
msrA	msr-F	TGCAAATGGCATACTATCGTC	160	32
	msr-R	CAAGAACGCTCAAGTGCTTC
PCR scanning
Region 1	region_1-F	CCTAAAATTGTTTGAATAGTATC	3,949	This study
	region_1-R	GGATTGAACTTCTGATAATCATT
Region 2	region_2-F	CTTGTGTCTTTTTATGTGGATTG	4,054	This study
	region_2-R	GACAATCTATTCATGATATAACT
Region 3	region_3-F	TTTATCAAGATAATCCCTTATCG	3,164	This study
	region_3-R	CACTTTTAAAATATGAACTAGGA
Region 4	region_4-F	TGTAAAGTATCTCTATTTTTAGC	3,150	This study
	region_4-R	CATTTAGGGGTATCTTATATATT
Region 5	region_5-F	CTTAGACCTTATTTAAAATATCC	2,019	This study
	region_5-R	CATAATTTTTGATAAAGTCCGTA
Region 6	region_6-F	AAATTTCTTTTCTACCATTTTCG	4,922	This study
	region_6-R	GTTAAAGATTTATTCCAACTACA
Region 7	region_7-F	ATTTAGATAGAAAAGAAAGAGCG	5,012	This study
	region_7-R	GATAAGCTTAAAGTAACTTCTTT